Sample Submission Forms
A variety of submission forms are available, please select the form that best suits your requirements. Forms can be printed out and submitted along with samples or sent electronically to the Unit at a.j.cassidy@dundee.ac.uk or s.e.reilly@dundee.ac.uk
DNA Sequencing Form
DNA Sequencing 16 sample form- Word Document
DNA Sequencing 16 sample form- PDF
DNA Sequencing 96 well form - PDF
DNA Sequencing 96 well form - Excel
Fragment Analysis Forms
Fragment Analysis 16 sample form- Word Document
Fragment Analysis 16 sample form- PDF
Real-Time PCR
Real-Time PCR 96 well plate form - PDF
Real-Time PCR 96 well plate form - Excel
Real-Time PCR 384 well plate form - Excel
DNA/RNA/Protein Analysis
Lab901 TapeStation Analysis Request-Word Document
Lab901 TapeStation Analysis Request-PDF
Mini-Projects
Project Request Form- Word Document
Submitting samples for DNA sequencing
Use 1.5 ml or 0.5 ml Eppendorf tubes for individual samples or 96-well plates for batch samples. Prepare your samples by adding the appropriate amount of template/primers to water (not TE or Tris buffers). Please supply 10ul of your template at a concentration based upon the table below. Please label your samples clearly.
Template |
Quantity/ul |
Primer/ul |
|---|---|---|
PCR Products: 100-200 bp 200-500 bp 500-1000 bp 1000-2000 bp >2000 bp |
1-3 ng 3-10 ng 5-20 ng 10-40 ng 20-50 ng |
5 pmol 5 pmol 5 pmol 5 pmol 5 pmol |
Single Stranded DNA |
25-50 ng |
5 pmol |
Double stranded DNA Plasmid |
150-300 ng |
5 pmol |
Cosmid, BAC DNA |
0.5-1.0 µg |
TBD |
Bacterial genomic DNA |
1 µg |
TBD |
Primer concentrations: we prefer 5 uM (5pmol/ul) in water.
Primer List
Click here to download a list of primers provided free of charge
We are also happy to add to this list if you have any suggestions.