Services Available
DNA Sequencing
DNA Sequencing by Capillary Electrophoresis is a key technology in a number of experimental and diagnostic workflows in the life science laboratory. We use the 3730 and 3130xl platforms to deliver our service.
DNA Concentration guidelines
Template |
Quantity/ul |
Primer/ul |
|---|---|---|
PCR Products: |
1-3 ng |
|
Single Stranded DNA |
25-50 ng |
5 pmol |
Double stranded DNA Plasmid |
150-300 ng |
5 pmol |
Cosmid, BAC DNA |
0.5-1.0 µg |
TBD |
Bacterial genomic DNA |
1 µg |
TBD |
Please provide at least 3ul/reaction for template and primer.
Fragment Analysis
Fragment analysis generates a size estimate for DNA fragments relative to a size standard of DNA fragments with known lengths. The size standard is combined with the sample of interest and co-injected on the capillary electrophoresis system. We use the 3730 and 3130xl platforms to deliver our service. Please contact us for details on sample requirements for fragment analysis.
Capillary Analyser Applications
De Novo Sequencing
BAC End Fingerprinting
Mitochondrial Sequencing
Fast Resequencing
Requencing/Heterozygote Detection
HLA Genotyping
AFLP® Analysis
Chimerism
ISSR (Inter-Simple Sequence Repeat) Analysis
Microsatellite Instability
Microsatellite/STR Analysis
Relative Fluorescent Quantification
RFLP Analysis
SNP Genotyping
SSCP Analysis
Methylation Analysis
Influenza A (H1N1) Sequencing
MSMSA (Methylation Sensitive Mobility Shift Assay)
Bacteria and Fungus Identification
Human Identification
Real-Time PCR
Our 7900HT System is a high-throughput real-time PCR system that detects and quantitates nucleic acid sequences. With 96 and 384-well plate capability the 7900HT system is ideally suited to meet the high-throughput requirements of today's drug discovery process. Key applications include gene expression quantitation and the detection of single nucleotide polymorphisms (SNPs) using the fluorogenic 5' nuclease assay.
DNA/RNA/Protein Analysis
The Lab901 ScreenTape system is a fully automated solution for gel electrophoresis that delivers analysed results in 1 minute per sample. The ScreenTape instrument loads, separates, images and analyses your samples, giving your more time for discovery.
Whether you perform DNA analysis, RNA analysis or protein analysis, Lab901 will change the way you do electrophoresis. Pre-packaged reagents and full automation mean safety, simplicity, speed and reproducibility.
Which ScreenTape to use
| Protein 10 to 200kDa | P200 |
| Total RNA | R6K |
| cRNA | R6K |
| DNA 25 to 800bp | D800 |
| DNA 125 to 1200bp | D1200 |
Mini-Projects
The Unit can provide a complete project service. If you have a project revolving around the equipment and techniques available in the Unit, we would be happy to undertake the project for you.